Pancreas preparation suitable for the treatment of diabetes.



ITNITED STATES PATENT OFFICE. enone ZuELZER, or IBERIIIN, ennmnn'xnssienon 'ro cHEiuIscHE nenmx AU]? I ACTIEN (vommn. scnname or BERLIN, GERMANY.

rANcRnAs PREPARATION sUITABLEron -'1HE TREATMENT on DIABETES.

No Drawing Specification of Letters Patent.

Application filed May 6, 190s. sum No. 431,226. (Specimens) A Patented May 28,1912.

To all whom it may concern) Be it known that I, GEoRo ZUELZER, doctor of medicine, citizen of the German'Empire, residing at Berlin, Kingdom of Prussia,

German Empire, have invented a new and useful Improvement in Pancreas Preparations Suitable for the Treatment of Diabetes, of which the following is a specification.

It is known that it is possible by injections of pancreas extract free from albumin to influence suprarenal diabetes produced experimentally. The preparation in question, however, was too septic to be used for persons.

According to this invention a non-poisonous preparation suitable for the treatment of natural diabetes is obtained from the pancreatic gland of an animal, which has been.

(cattle, pigs, sheep, dogs, &c.) in narcosis is laid bare, the animaljbeing preferably. in astate when the process of digestion is at its height, the principal efferent vein is preparedandligatured directly in front of it entry into the portal-vein system. In a Simillar manner the remaining veins of the pancreas are tied and swell greatly after the first ligature. After one to. two hours the pancreas is removed for further treatment. This treatment consists in cutting it up into small pieces, leaving the same to self-digestion' under weak alkaline reaction for several days, although this self-digestion may be omitted, then precipitating the albuminous bodies, by means of alcohol or by boiling, preferably under the addition of common salt, whereby ferments such as trypsin, steapsin, diastase, &c., are d troyed, and then evaporat'ng the filtrate; dry preparati 's a fine, gray,

ingpowder, Which is easily soluble in water or dllute soda solution, is free from ferments and init's best form gives none ofth'e known p'rotem reactions. ThlS dry preparation or:

residueis the product which is produced according to this invention. in its best form but it is obvious that the same product may also be-employed in the form ofa solution since the product is easilysoluble in Water or dilute soda solution. The process is carriedout in-a similar manner if in place ofboth only one of the above stated conditions is fulfilled, but the preparation so obtained will be less rich in active substance.

Example: The pancreas is chopped fine o minced in a meat-chopping machine and in order to neutralizea'ny organic acid which may be formed, suflicient sodium bicarbonate is added to produce a weak alkaline, reaction.

About 2 to 5 cubic centimeters of aj nor-' mal sodasolution may be used. The chopped pancreas is then left to self-digestion for several days, then pressed out under high pres sure, alcohol added to the liquid so long as; albuminous substances are precipitated, and

the liquid boiledunder the addition of common salt. When the solution is free from albumin it is filtered and evaporated in vac a0. If there is any danger of the pancreas spoiling during the digestive process, this can be prevented by adding a solution of common salt, preferably a four per cent. solution. 0.2 to 0.3 gram of the dry substance represent a unit which injected hypodermically neutralizes one milligram'of adrenalin. In the treatment of human beings from .4: to

6 units may be injected at a time according i to the nature of the case.

No specific reactions with chemical re-. agents have been observed. As compared with adrenalin the herein described pancreas preparation is not permanent in acidsolution. The preparation does not lose its efiiciency byboiling and-can be applied-in the} form of'a solution, preferably wlth commonsalt.

larly suitable for the treatment of diabetes and is administered advantageously by injecting the same into the venous system, preferably diluted by means of a physiological solution of common salt. For illustration, if

adrenalin, also known as-suprarenin, is injected into an animala condition is produced during which .su'gar occurs in the urine.

The above described, product is particulot This condition'is changed by the injection H of the above described product in such manner that the sugar disappears. There appears to be anantagonism between a cause or condition whichlmanifests itself by sugar in the urine and this product, similar to that which exists between toxin and antitoxin.

I claim as my invention:

1. The herein described pancreas preparation having the property of antagonizing a cause or condition which manifests itself by sugar in the urine, said preparation being, When in a dry state, a fine, gray, feebly smelling powder, easily soluble in Water or dilute soda solution, free from ferments, and giving none of the known protein reactions, substantially as set forth.

2. The herein described process of manufacturing a pancreas preparation having the property of antagonizing a cause or condition which manifests itself by sugar in the urine, which consists in removing the pan- 'creas from suitable animals, mincin the same, extracting the liquid from the minced pancreas, precipitating the albuminous substances from said liquid and evaporating the filtrate, substantially as set forth.

3. The herein described process of manufacturmg a pancreas preparation having the property of antagonizing a cause or condition which manifests itself by sugar in the urine, which consists in removing the pancreas from suitable animals, leaving the pancreas to self-digestion, extracting the liquid, precipitating the albuminous substances contained therein and evaporating the filtrate, substantially asset forth.

4. The herein described process of manufacturing a pancreas preparation having the property of anta onizing a cause or condition which manifests itself by sugar in the urine, which consists in enriching the pancreas in the animal by artlficial stasis, then removing the pancreas from the animal, leaving the pancreas to self-digestion, extracting the liquid, precipitating the albuminous substances contained therein and evaporating the filtrate, substantially as set forth.

In testimony whereof I have hereunto set my hand in the presence of tWo subscribing Witnesses.

GEORG ZUELZER.

Witnesses W HENRY HAsPnR, EMIL GRUNWALD. 

